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Portraitfoto Bastmeyer
Spokesperson Thrust C3
Prof. Dr. Martin Bastmeyer
Karlsruhe Institute for Technology (KIT)

bastmeyer∂kit edu

Portraitfoto Wittbrodt
Spokesperson Thrust C3
Prof. Dr. Joachim Wittbrodt
Heidelberg University

jochen wittbrodt∂cos uni-heidelberg de

3D Hybrid Organotypic Systems (C3)

Thrust C3 intends to design organ functionality in a petri dish through 3D printing technologies.

Recent progress in combining surface functionalization and microfabrication with advanced tissue culture methods and genomic engineering has led to the perspective of designing organ functionality in a petri dish through printing technologies. Thrust C3 will realize next-generation 3D scaffolds to direct the assembly of 3D organotypic systems starting from pluripotent stem cells. We have selected the mammalian retina as a model system, since it is the best understood vertebrate neuronal sensory organ with all cell types, their respective connectomes, and individual gene expression profiles being elucidated.

Starting with mouse embryonic stem cells, we will use a combination of scaffold printing developed in Thrusts B1 and B2 with organoid cell culture approaches.  Given the expertise of PIs in Thrust C3, we will initially understand the basic biological and biophysical mechanisms underlying retinal stem cell division, migration, differentiation, and synapse formation within the artificial scaffold. Our approach will be supported by tailored chemistry developed in Thrusts A1-A3.  This will provide us with novel strategies for selective bio-functionalization techniques and adaptive materials that change their properties (size, biodegradability, stiffness) upon the application of external stimuli.

Re-designed Retina
Figure: Re-designed Retina

In a three-step process, we will understand the biological and biophysical building blocks, construct new tools and techniques to direct the growth and differentiation of stem cells towards an ordered and functional 3D neuronal tissue, and ultimately apply our combined expertise for hybrid disease models and drug discovery tools.