IMT 09-19 Master Thesis: Fabriction of an amperometric glucose biosensor via chitosan electrodeposition on a laser carbon electrode
Final Thesis (Bachelor and Master Thesis)
Many phenolic resins undergo transformation to carbon by applied heating in an inert environment, which is called pyrolysis. However, some of the resins also have the tendency to carbonize/pyrolyze under laser radiation with short pulse duration, one of such polymers is polyimide (commercially known as Kapton). Such laser carbons attain properties different from typical pyrolyzed carbons: with substantial increase in porosity, conductivity and presence of hydroxyl, carboxyl and carbonyl surface functional groups.
Those properties are favourable for the deposition of the bio-polymer chitosan, derived by deacetylation of the chitin from the shells of crustaceans. Chitosan has several advantages due to its biocompatibility and degradability, it can be easily deposited as a hydrogel on conductive substrates via electrochemical processes and thus functions as a perfect substrate for enzyme immobilization and biosensors that are integrated into flexible substrates.[1,2]
At the Institute for Microstructure Technology (IMT) we focus on the investigation of the fundamental properties of both materials – laser carbon and chitosan, for the utilization in biosensors. Previously, it was successfully shown that deposition of chitosan and subsequent immobilization of the enzyme urease yielded an integrated biosensor for urea detection within a catheter tube. Our research interest extends to the fabrication of additional enzyme-based biosensors.
The work will involve tasks including:
Design and fabrication of the carbon substrates and utilization as biosensors;
Demonstrate the functionalization of the immobilization enzyme;
Characterisation of the fabricated substrates/devices with FT-infrared spectrometry, cyclic voltammetry, X-ray photoelectron spectroscopy.
You will be part of a larger team within the Institute of Microstructure Technology (IMT), which offers you the possibility to fabricate and test the devices, and gives you the opportunity to co-author conference and/or journal publications.
 E. R. Mamleyev, S. Heissler, A. Nefedov, P. G. Weidler, N. Nordin, V. V. Kudryashov, K. Länge, N. MacKinnon and S. Sharma, "Laser-induced hierarchical carbon patterns on polyimide substrates for flexible urea sensors," npj Flexible Electronics, vol. 3, no. 1, p. 2, 2019.
 E. R. Mamleyev, N. Nordin, S. Heissler, K. Länge, N. MacKinnon and S. Sharma, "Flexible Carbon-based Urea Sensor by Laser Induced Carbonisation of Polyimide," in 2018 International Flexible Electronics Technology Conference (IFETC). IEEE, Ottawa, 2018.
Further information on the Institute of Microstructure Technology (IMT): www.imt.kit.edu
Experience in the software: Origin Pro, CorelDraw, MatLAB;
Interest in electro-chemistry, physical chemistry and bio-chemistry;
Enthusiasm to work independently in multidisciplinary, multicultural environment.
Field of study: Material Science, Physical chemistry, Bio-chemical engineering, Electrical engineering